Rejoining of DNA by the RAG1 and RAG2 Proteins

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Science  10 Apr 1998:
Vol. 280, Issue 5361, pp. 301-303
DOI: 10.1126/science.280.5361.301

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Assembly of immunoglobulin and T cell receptor genes from separate gene segments [V(D)J recombination] begins with DNA double-strand breakage by the RAG1 and RAG2 proteins, acting at a pair of recombination signal sequences (RSSs). Here, the RAG proteins are shown to reverse the cleavage reaction by joining an RSS to a broken coding sequence end. These “hybrid joints” have also been found in lymphoid cells, even when the normal pathway of DNA double-strand break repair is inactive, and can now be explained by this activity of the RAG proteins.

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