Report

In Situ Visualization of DNA Double-Strand Break Repair in Human Fibroblasts

Science  24 Apr 1998:
Vol. 280, Issue 5363, pp. 590-592
DOI: 10.1126/science.280.5363.590

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Abstract

A method was developed to examine DNA repair within the intact cell. Ultrasoft x-rays were used to induce DNA double-strand breaks (DSBs) in defined subnuclear volumes of human fibroblasts and DNA repair was visualized at those sites. The DSBs remained in a fixed position during the initial stages of DNA repair, and the DSB repair protein hMre11 migrated to the sites of damage within 30 minutes. In contrast, hRad51, a human RecA homolog, did not localize at sites of DNA damage, a finding consistent with the distinct roles of these proteins in DNA repair.

  • * These authors contributed equally to this work. Names are listed in random order.

  • To whom correspondence should be addressed. E-mail: jpetrini{at}facstaff.wisc.edu

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