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Mixer, a Homeobox Gene Required for Endoderm Development

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Science  03 Jul 1998:
Vol. 281, Issue 5373, pp. 91-96
DOI: 10.1126/science.281.5373.91

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Abstract

An expression cloning strategy in Xenopus laevis was used to isolate a homeobox-containing gene, Mixer, that can cause embryonic cells to form endoderm. Mixer transcripts are found specifically in the prospective endoderm of gastrula, which coincides with the time and place that endodermal cells become histologically distinct and irreversibly determined. Loss-of-function studies with a dominant inhibitory mutant demonstrate thatMixer activity is required for endoderm development. In particular, the expression of Sox17α andSox17β, two previously identified endodermal determinants, require Mixer function. Together, these data suggest that Mixer is an embryonic transcription factor involved in specifying the endodermal germ layer.

  • * To whom correspondence should be addressed. E-mail: dmelton{at}biohp.harvard.edu

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