Fusion Reconstituted

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Science  31 Jul 1998:
Vol. 281, Issue 5377, pp. 617
DOI: 10.1126/science.281.5377.617k

Targeted membrane fusion occurs during intracellular transport, for example, after endocytosis when endocytic vesicles fuse to form endosomes and lysosomes. In yeast, the functional equivalent of the lysosome is the vacuole. Using solubilized vacuole membrane components, Sato and Wickner (p. 700) were able to reconstitute experimentally the fusion process having the appropriate energy and temperature characteristics. They could then dissect the importance of particular proteins in the simplified fusion system.

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