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Determinants of Kinesin Motor Polarity

Science  21 Aug 1998:
Vol. 281, Issue 5380, pp. 1200-1202
DOI: 10.1126/science.281.5380.1200

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Abstract

The kinesin motor protein family members move along microtubules with characteristic polarity. Chimeric motors containing the stalk and neck of the minus-end-directed motor, Ncd, fused to the motor domain of plus-end-directed kinesin were analyzed. The Ncd stalk and neck reversed kinesin motor polarity, but mutation of the Ncd neck reverted the chimeric motor to plus-end movement. Thus, residues or regions contributing to motor polarity must be present in both the Ncd neck and the kinesin motor core. The neck-motor junction was critical for Ncd minus-end movement; attachment of the neck to the stalk may also play a role.

  • * To whom correspondence should be addressed. E-mail: endow{at}galactose.mc.duke.edu

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