Research Article

A Preorganized Active Site in the Crystal Structure of the Tetrahymena Ribozyme

Science  09 Oct 1998:
Vol. 282, Issue 5387, pp. 259-264
DOI: 10.1126/science.282.5387.259

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Abstract

Group I introns possess a single active site that catalyzes the two sequential reactions of self-splicing. An RNA comprising the two domains of the Tetrahymena thermophila group I intron catalytic core retains activity, and the 5.0 angstrom crystal structure of this 247-nucleotide ribozyme is now described. Close packing of the two domains forms a shallow cleft capable of binding the short helix that contains the 5′ splice site. The helix that provides the binding site for the guanosine substrate deviates significantly from A-form geometry, providing a tight binding pocket. The binding pockets for both the 5′ splice site helix and guanosine are formed and oriented in the absence of these substrates. Thus, this large ribozyme is largely preorganized for catalysis, much like a globular protein enzyme.

  • * To whom correspondence should be addressed. E-mail: bgolden{at}petunia.colorado.edu (B.L.G.); Thomas.Cech{at}colorado.edu(T.R.C.).

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