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Defective LPS Signaling in C3H/HeJ and C57BL/10ScCr Mice: Mutations in Tlr4 Gene

Science  11 Dec 1998:
Vol. 282, Issue 5396, pp. 2085-2088
DOI: 10.1126/science.282.5396.2085

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Abstract

Mutations of the gene Lps selectively impede lipopolysaccharide (LPS) signal transduction in C3H/HeJ and C57BL/10ScCr mice, rendering them resistant to endotoxin yet highly susceptible to Gram-negative infection. The codominantLps d allele of C3H/HeJ mice was shown to correspond to a missense mutation in the third exon of the Toll-like receptor-4 gene (Tlr4), predicted to replace proline with histidine at position 712 of the polypeptide chain. C57BL/10ScCr mice are homozygous for a null mutation of Tlr4. Thus, the mammalian Tlr4 protein has been adapted primarily to subserve the recognition of LPS and presumably transduces the LPS signal across the plasma membrane. Destructive mutations of Tlr4 predispose to the development of Gram-negative sepsis, leaving most aspects of immune function intact.

  • * Present address: Northwestern University, 2300 Children's Plaza, No. 209, Chicago, IL 60614–3394, USA.

  • Present address: University of Texas Southwestern Medical Center, Department of Pharmacology, Dallas, TX 75235–9041 USA.

  • Present address: Millennium, Inc., Cambridge, MA 02139–4815, USA.

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