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Silencing of Genes Flanking the P1 Plasmid Centromere

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Science  22 Jan 1999:
Vol. 283, Issue 5401, pp. 546-549
DOI: 10.1126/science.283.5401.546

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Abstract

Partition modules stabilize bacterial plasmids and chromosomes by actively promoting their segregation into daughter cells. The partition module of plasmid P1 is typical and consists of a centromere site,parS, and genes that encode proteins ParA and ParB. We show that ParB can silence genes flanking parS (to which ParB binds), apparently by polymerizing along the DNA from a nucleation site at parS. Wild-type ParB contacts an extensive region of P1 DNA; silencing-defective ParB proteins, which were found to be partition-defective, are less able to spread. Hence, the silenced structure appears to function in partitioning.

  • To whom correspondence should be addressed. E-mail: myarmo{at}helix.nih.gov

  • * Present address: Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ul. Pawińskiego 5A, 02-106, Warsaw, Poland.

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