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Single-Channel Recording of a Store-Operated Ca2+ Channel in Jurkat T Lymphocytes

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Science  05 Feb 1999:
Vol. 283, Issue 5403, pp. 836-839
DOI: 10.1126/science.283.5403.836

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Abstract

In T lymphocytes, a store-operated calcium ion (Ca2+) entry mechanism termed the calcium release–activated Ca2+ channel (CRAC channel) underlies the sustained or oscillatory intracellular calcium concentration signal required for interleukin-2 gene expression and cell proliferation. The use of sodium ions as a current carrier enabled single-channel recordings of CRAC channels during activation, inactivation, and blockade of current in the presence of divalent cations. A large conductance of 36 to 40 picosiemens indicates that 100 to 400 CRAC channels are present in T lymphocytes.

  • * To whom correspondence should be addressed. E-mail: mcahalan{at}uci.edu

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