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Efficient Bypass of a Thymine-Thymine Dimer by Yeast DNA Polymerase, Polη

Science  12 Feb 1999:
Vol. 283, Issue 5404, pp. 1001-1004
DOI: 10.1126/science.283.5404.1001

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Abstract

The RAD30 gene of the yeast Saccharomyces cerevisiae is required for the error-free postreplicational repair of DNA that has been damaged by ultraviolet irradiation. Here,RAD30 is shown to encode a DNA polymerase that can replicate efficiently past a thymine-thymine cis-syn cyclobutane dimer, a lesion that normally blocks DNA polymerases. When incubated in vitro with all four nucleotides, Rad30 incorporates two adenines opposite the thymine-thymine dimer. Rad30 is the seventh eukaryotic DNA polymerase to be described and hence is named DNA polymerase η.

  • * To whom correspondence should be addressed. E-mail: lprakash{at}scms.utmb.edu

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