Peptide Bond Formation: Retraction

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Science  26 Mar 1999:
Vol. 283, Issue 5410, pp. 2017
DOI: 10.1126/science.283.5410.2017g

We recently reported that N-acetylphenylalanylphenylalanine (AcPhe-Phe) was produced from the peptidyl-transfer RNA (tRNA) analog N-acetylphenylalanyl- tRNA (AcPhe-tRNA) and phenylalanyl-tRNA (Phe-tRNA) in the presence of the entire 23S ribosomal RNA (rRNA) or with domain V alone prepared by in vitro transcription (Research Article, 31 July, p. 666) (1, 2). However, we subsequently discovered that there were problems with the identification of the products by thin-layer chromatography (TLC). We (3) and Khaitovich et al. (4) found independently that the spot on the TLC plate that we previously identified as AcPhe-Phe consisted mainly of AcPhe-methyl- or ethyl-ester (AcPhe-OMe or AcPhe-OEt), which might have been produced by the reaction of AcPhe-tRNA and residual alcohol (0.1% or less) in the RNA preparations.

Once we discovered this product misidentification on the TLC plates, we realized that the data in our Science paper concerning quantitative analysis of the spot were not definitive.

In light of these observations, we would like to retract our Science paper (1), pending further experimentation and resolution of the ambiguities. We apologize to our colleagues for any trouble caused by this error.


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