Report

Phenotypic Change Caused by Transcriptional Bypass of Uracil in Nondividing Cells

Science  02 Apr 1999:
Vol. 284, Issue 5411, pp. 159-162
DOI: 10.1126/science.284.5411.159

You are currently viewing the abstract.

View Full Text
As a service to the community, AAAS/Science has made this article free with registration.

Abstract

Cytosine deamination to uracil occurs frequently in cellular DNA. In vitro, RNA polymerase efficiently inserts adenine opposite to uracil, resulting in G to A base substitutions. In vivo, uracil could potentially alter transcriptional fidelity, resulting in production of mutant proteins. This study demonstrates that in nondividingEscherichia coli cells, a DNA template base replaced with uracil in a stop codon in the firefly luciferase gene results in conversion of inactive to active luciferase. The level of transcriptional base substitution is dependent on the capacity to repair uracil. These results provide evidence for a DNA damage–dependent, transcription-driven pathway for generating mutant proteins in nondividing cells.

  • * To whom correspondence should be addressed. E-mail: medpwd{at}emory.edu

View Full Text

Cited By...