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Xyloglucan Fucosyltransferase, an Enzyme Involved in Plant Cell Wall Biosynthesis

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Science  18 Jun 1999:
Vol. 284, Issue 5422, pp. 1976-1979
DOI: 10.1126/science.284.5422.1976

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Abstract

Cell walls are crucial for development, signal transduction, and disease resistance in plants. Cell walls are made of cellulose, hemicelluloses, and pectins. Xyloglucan (XG), the principal load-bearing hemicellulose of dicotyledonous plants, has a terminal fucosyl residue. A 60-kilodalton fucosyltransferase (FTase) that adds this residue was purified from pea epicotyls. Peptide sequence information from the pea FTase allowed the cloning of a homologous gene, AtFT1, from Arabidopsis. Antibodies raised against recombinant AtFTase immunoprecipitate FTase enzyme activity from solubilized Arabidopsis membrane proteins, andAtFT1 expressed in mammalian COS cells results in the presence of XG FTase activity in these cells.

  • * These authors contributed equally to this work.

  • Present address: Monsanto, St. Louis, MO 63198, USA.

  • To whom correspondence should be addressed at MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA. E-mail: nraikhel{at}pilot.msu.edu (N.V.R.); keegstra{at}pilot.msu.edu (K.K.)

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