Research Article

Archean Molecular Fossils and the Early Rise of Eukaryotes

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Science  13 Aug 1999:
Vol. 285, Issue 5430, pp. 1033-1036
DOI: 10.1126/science.285.5430.1033

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Abstract

Molecular fossils of biological lipids are preserved in 2700-million-year-old shales from the Pilbara Craton, Australia. Sequential extraction of adjacent samples shows that these hydrocarbon biomarkers are indigenous and syngenetic to the Archean shales, greatly extending the known geological range of such molecules. The presence of abundant 2α-methylhopanes, which are characteristic of cyanobacteria, indicates that oxygenic photosynthesis evolved well before the atmosphere became oxidizing. The presence of steranes, particularly cholestane and its 28- to 30-carbon analogs, provides persuasive evidence for the existence of eukaryotes 500 million to 1 billion years before the extant fossil record indicates that the lineage arose.

Microfossils (1), stromatolites (2), and sedimentary carbon isotope ratios (3) all indicate that microbial organisms inhabited the oceans in Archean times [>2500 million years ago (Ma)]. But these lines of evidence are not very informative about what these microbes were or how they lived. Potentially, a better insight into primordial biological diversity can be obtained from molecular fossils derived from cellular and membrane lipids (“biomarkers”). Although such soluble hydrocarbons were first extracted from Archean rocks more than 30 years ago, their significance was generally discounted after amino acids of recent origin were found in the same rocks (4). Prevailing models of thermal maturation dictated that complex hydrocarbons should not survive the metamorphism experienced by all Archean terrains. However, indications of greater hydrocarbon stability (5) and observations of oil in Archean fluid inclusions (6) suggest that these maturation models are unduly pessimistic and that biomarkers could indeed be preserved in low-grade Archean metasedimentary rocks. Furthermore, systematic sampling strategies, improved analytical techniques, and greater geochemical knowledge (7) should make their recognition easier and their interpretation more rigorous. We now report molecular fossils in late Archean shales that have suffered only minimal metamorphism. These molecular fossils reveal that the Archean biota was considerably more complex than currently recognized and that the domains Eucarya and Bacteria were already extant.

Samples came from depths of around 700 m in diamond drill core WRL#1, collared near Wittenoom in the Pilbara Craton of northwestern Australia (8). They represent the ∼2600-Ma Marra Mamba Formation (9) (lowermost Hamersley Group), the underlying Roy Hill and Warrie Members of the ∼2690-Ma Jeerinah Formation (10), and the ∼2715-Ma Maddina Formation (Fortescue Group) (9), all of which have only been metamorphosed to prehnite-pumpellyite facies in this area (11) (∼200° to ∼300°C). The closely spaced sampling of different lithologies allowed comparison of rocks with varying compositions, porosities, and kerogen contents but with identical postdepositional histories. Most analyses were of finely laminated kerogenous shales from the Roy Hill Shale and Marra Mamba Formation that were deposited in a marine continental-slope environment below storm-wave base under suboxic conditions (12). Those from the Marra Mamba Formation were interbedded with oxide-facies banded iron formation. Black chert from the Warrie Member, quartz sandstone and vein dolomite from the Roy Hill Member, and terrestrial basalt from the Maddina Formation served as controls for laboratory procedures.

Although these rocks are well-preserved by Archean standards, they are nevertheless highly mature and therefore contain only small quantities of extractable hydrocarbons. Hence, they are especially prone to modern contamination by petroleum products during drilling, storage, and analysis. Accordingly, each sample underwent three solvent rinses to ensure that extracted bitumen was intimately associated with the rock and not superficial or lining cracks. After each rinse was analyzed chromatographically, the rock was broken into smaller fragments to expose fresh bedding surfaces, fractures, and fissures. If the last rinse proved clean, the sample was then finely ground and exhaustively extracted. The kerogenous shales yielded >25 μg of saturated hydrocarbons per gram of rock, 50 to 500 times more than the concentrations obtained from the chert and basalt and 50,000 times greater than laboratory system blanks (Table 1). The low hydrocarbon contents of the sandstone and vein dolomite represent traces of oil expelled from directly adjacent shales. Because modern petrochemical contamination should produce similar hydrocarbon distributions in all samples, the marked variations in these (Fig. 1) indicate that the extracted hydrocarbons are indeed indigenous.

Figure 1

Drill core WRL#1 showing the gas chromatograms of extractable hydrocarbons at different depths (in meters). The kerogen-poor samples on the left (white squares on drill core) yielded low quantities of extractable organic matter, whereas the kerogen-rich shales on the right (black squares on drill core) yielded relatively high concentrations.

Table 1

Kerogen and bitumen contents of WRL#1 samples. TOC, total organic carbon. n.m., not measured.

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There are three potential sources of ancient but post-Archean organic contamination: local subsurface biological activity, groundwater carrying biolipids from the surface, and migrating petroleum carrying geolipids. The first two are easily excluded. Hydrous pyrolysis at 350°C of kerogens isolated from the shales Roy3 and Mam1 yielded insignificant quantities of hydrocarbon, as expected from highly mature Precambrian organic matter (13). Hydrocarbons extracted from these kerogens are also highly mature, based on the saturate and aromatic biomarker parameters conventionally used to assess thermal evolution (Table 2). This indicates that they were generated during the final stages of kerogen maturation, before or during peak metamorphism at 2450 to 2000 Ma (14). Because the rocks have not experienced a subsequent thermal event capable of turning immature biolipids into highly mature geolipids, the extracted hydrocarbons were not derived from post-Archean surface or subsurface biota.

Table 2

Abundances and isotopic compositions of kerogens and individual compounds. Biomarker ratios were measured according to published methods (7, 15). Numbers in subscript refer to the number of carbon atoms in the biomarker.

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Adulteration from migrating Phanerozoic or Proterozoic petroleum is harder to discount. The youngest rocks in the depositional basin belong to the ∼2400-Ma Turee Creek Group. Younger petroleum-prone rocks were never deposited over the top of the basin in sufficient thickness for hydrocarbon expulsion to occur. The WRL#1 drill hole is located centrally within the craton, at least 150 km from the nearest post-Archean basin, so younger oils migrating laterally would have to travel long distances through deformed and metamorphosed Archean rocks without continuous cross-cutting fractures. Thus, the only likely source for adulterating oil is from older, rather than younger, rocks. Moreover, bedding-parallel permeability of the black shale Roy3 and pyritic sandstone Roy4 was below the detection limit of 0.01 millidarcy. Thus, these rocks might have been sealed to hydrocarbon migration since they were last metamorphosed at 2450 to 2000 Ma. Also, higher yields of bitumen from shales than from kerogen-poor rocks (Table 1, Fig. 1) are inconsistent with staining by migrated oil, which would have equally tainted all rocks of similar permeability. Notably, typical Phanerozoic geochemical characters, such as biomarkers derived from higher plants, are absent. However, isoalkanes are abundant, as in many Proterozoic bitumens (15,16), and the n-alkane distribution has a diminution in relative abundance at the n-C22homolog (Fig. 1), a pattern not observed in younger bitumens.

The strongest argument for hydrocarbon syngeneity is the variation in biomarker distributions between closely spaced shale samples (Table 2). The sterane:hopane ratio and 2α-methylhopane index are both gauges of different biological inputs during sedimentation, as are the ratios of sterane and hopane homologs. These differences among biomarker ratios cannot be explained by water washing, biodegradation, thermal degradation, or geochromatography of a homogeneous migrated oil. The observed variation is instead consistent with the diversity of syngenetic bitumen typically present within source rock facies.

To provide clues about organic origins, carbon isotopic ratios were measured for bulk kerogen and for individual hydrocarbons. Kerogens are depleted in 13C as is typical of rocks of this age, with values of δ13C falling between −40 and −47 per mil (Table 2). The δ13C values for 12- to 22-carbon (C12-C22) n-alkanes range from −26 to −29 per mil. This enrichment in 13C relative to kerogen is very rare in Phanerozoic kerogen-bitumen pairs but typical of, though more extreme than, other Precambrian samples (17,18)—for example, the 600-Ma Pertatataka Formation where C16-C20 n-alkanes are up to 6 per mil heavier than kerogen (19). It indicates that the organisms forming most of the kerogen did not contribute significantly to the n-alkanes. The isoprenoids pristane and phytane are depleted in 13C relative to n-alkyl C17 and C18 compounds by ∼3 per mil (Table 2), as in other analyses of Precambrian bitumen (18). Then-alkanes are probably the products of a diverse biota of primary producers and heterotrophs. The isoprenoids are derived from photosynthetic microbes with a possible contribution from isotopically light methanogenic Archaea, which are known to yield only small quantities of pristane and phytane relative to total biomass and non-alkanes when pyrolyzed (20). The contrasting depletion of 13C in the kerogen may be attributed to contributions from methanotrophs, as proposed by Hayes (21). However, n-alkanes derived from isotopically depleted membrane lipids of methanotrophs could not be identified in our samples. Furthermore, we cannot discount the possibility that the observed isotopic pattern could also reflect the products of an extinct biochemistry.

More information about biological precursors can be obtained from the tetracyclic and pentacyclic terpane biomarkers in the hydrocarbon extracts (Fig. 2). Until now, evidence for cyanobacteria in the Archean has been equivocal, based on poorly preserved microfossils (22) and on indirect geochemical arguments (23). High concentrations of 2α-methylhopanes (Table 2), also observed in other Precambrian and early Paleozoic sediments and apparently of cyanobacterial origin (24), are consistent with the early appearance of these organisms. Since oxygenic photosynthesis is their preferred physiology, metabolic oxygen excretion was evidently occurring well before significant oxygen had accumulated in the atmosphere, at about 2000 Ma (25). The abundance of cyanobacterial biomarkers in the Marra Mamba Formation, a unit predominantly consisting of oxide-facies banded iron formation, suggests that although Precambrian iron formations could have been produced by abiotic photochemical processes (26) or anoxygenic phototrophic bacteria (27), those in the Hamersley Group probably formed as a result of biogenic oxygen production (28).

Figure 2

Distribution of (A) sterane and (B) hopane hydrocarbons from Roy1. The data are MRM (multiple reaction monitoring) chromatograms obtained by gas chromatography–mass spectrometry (GC-MS) analysis of metastable molecular ions fragmenting to daughter ions at mass-to-charge ratio (m/z) = 217 for steranes,m/z = 191 for hopanes, andm/z = 205 for methylhopanes. Chromatograms are identified by carbon numbers, the relative height (abundance) of the most intense peak in the trace, and the reaction transition.

Steranes also occur in the extracted bitumens (Fig. 2). They comprise most of the known C26 to C30pseudohomologs, their aromatic counterparts, and A-ring methylated steranes that have been recorded in younger sediments. The biosynthesis of these sterols is characteristic of eukaryotes. The only prokaryotes known to synthesize sterols have biosynthetic pathways that stop short of cholesterol (29) or exclusively produce C27 cholestenols (30). Although it is possible that other yet unknown or extinct prokaryotes also produced sterols, the wide structural range of steranes, in relative abundances like those of younger bitumen, is convincing evidence for the presence of eukaryotes in the late Archean. The phylogenetic position of these eukaryotes remains unclear. The curtailed n-alkane distribution indicates that contributions from polymethylenic chains, derived from algal cell wall components (algaenans), were much lower than observed in younger marine sediments or were missing. We conclude that the domain Eucarya first appeared before 2700 Ma and is at least 500 to 1000 My older than indicated by current paleontological data (31). This age should provide a new calibration point for molecular clocks and the universal tree of life.

The biomarkers we report are the oldest known that are demonstrably indigenous and syngenetic. They are more than a billion years older than those from the ∼1640-Ma Barney Creek Formation (15), previously the oldest well-characterized molecular fossils. Their distinctive features are very similar to those observed in the ∼2500-Ma Mt. McRae Shale, and their age is supported by more thorough analytical protocols (24). The discovery and careful analysis of biomarkers in rocks of still greater age and of different Archean environments will potentially offer new insights into early microbial life and its evolution.

  • * To whom correspondence should be addressed. E-mail: jochen.brocks{at}agso.gov.au or brocks{at}es.su.oz.au

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