AP-2 Recruitment to Synaptotagmin Stimulated by Tyrosine-Based Endocytic Motifs

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Science  20 Aug 1999:
Vol. 285, Issue 5431, pp. 1268-1271
DOI: 10.1126/science.285.5431.1268

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Clathrin-mediated endocytosis is initiated by the recruitment of the clathrin adaptor protein AP-2 to the plasma membrane where the membrane protein synaptotagmin is thought to act as a docking site. AP-2 also interacts with endocytic motifs present in other cargo proteins. Peptides with a tyrosine-based endocytic motif stimulated binding of AP-2 to synaptotagmin and enhanced AP-2 recruitment to the plasma membrane of neuronal and non-neuronal cells. This suggests a mechanism by which nucleation of clathrin-coated pits is stimulated by the loading of cargo proteins.

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