Whole-Genome Shotgun Optical Mapping of Deinococcus radiodurans

Science  03 Sep 1999:
Vol. 285, Issue 5433, pp. 1558-1562
DOI: 10.1126/science.285.5433.1558

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A whole-genome restriction map of Deinococcus radiodurans, a radiation-resistant bacterium able to survive up to 15,000 grays of ionizing radiation, was constructed without using DNA libraries, the polymerase chain reaction, or electrophoresis. Very large, randomly sheared, genomic DNA fragments were used to construct maps from individual DNA molecules that were assembled into two circular overlapping maps (2.6 and 0.415 megabases), without gaps. A third smaller chromosome (176 kilobases) was identified and characterized. Aberrant nonlinear DNA structures that may define chromosome structure and organization, as well as intermediates in DNA repair, were directly visualized by optical mapping techniques after γ irradiation.

  • * Present address: Genome Therapeutics Corp., 100 Beaver Street, Waltham, MA 02154, USA.

  • Present address: CNS Disorders, Wyeth-Ayerst Research, Princeton, NJ 08543, USA.

  • Present address: Smith Kline Beecham Pharmaceuticals, King of Prussia, PA 19406, USA.

  • § To whom correspondence should be addressed. Present address: Departments of Chemistry and Genetics, UW Biotechnology Center, University of Wisconsin, 425 Henry Mall, Madison, WI 53706, USA. E-mail: schwad01{at}

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