Report

An Adenosine Deaminase that Generates Inosine at the Wobble Position of tRNAs

Science  05 Nov 1999:
Vol. 286, Issue 5442, pp. 1146-1149
DOI: 10.1126/science.286.5442.1146

You are currently viewing the abstract.

View Full Text

Via your Institution

Log in through your institution

Log in through your institution


Abstract

Several transfer RNAs (tRNAs) contain inosine (I) at the first position of their anticodon (position 34); this modification is thought to enlarge the codon recognition capacity during protein synthesis. The tRNA-specific adenosine deaminase of Saccharomyces cerevisiae that forms I34 in tRNAs is described. The heterodimeric enzyme consists of two sequence-related subunits (Tad2p/ADAT2 and Tad3p/ADAT3), both of which contain cytidine deaminase (CDA) motifs. Each subunit is encoded by an essential gene (TAD2 and TAD3), indicating that I34is an indispensable base modification in elongating tRNAs. These results provide an evolutionary link between the CDA superfamily and RNA-dependent adenosine deaminases (ADARs/ADATs).

  • * To whom correspondence should be addressed. E-mail: walter.keller{at}unibas.ch

View Full Text

Related Content