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The Protein Kinase p90 Rsk as an Essential Mediator of Cytostatic Factor Activity

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Science  12 Nov 1999:
Vol. 286, Issue 5443, pp. 1362-1365
DOI: 10.1126/science.286.5443.1362

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Abstract

Persistent activation of p42 mitogen-activated protein kinase (p42 MAPK) during mitosis induces a “cytostatic factor” arrest, the arrest responsible for preventing the parthenogenetic activation of unfertilized eggs. The protein kinase p90 Rsk is a substrate of p42 MAPK; thus, the role of p90 Rsk in p42 MAPK-induced mitotic arrest was examined. Xenopus laevis egg extracts immunodepleted of Rsk lost their capacity to undergo mitotic arrest in response to activation of the Mos–MEK-1–p42 MAPK cascade of protein kinases. Replenishing Rsk-depleted extracts with catalytically competent Rsk protein restored the ability of the extracts to undergo mitotic arrest. Rsk appears to be essential for cytostatic factor arrest.

  • * To whom correspondence should be addressed. E-mail: ferrell{at}cmgm.stanford.edu

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