Report

Evidence for Autoregulation of Cystathionine γ-Synthase mRNA Stability in Arabidopsis

+ See all authors and affiliations

Science  12 Nov 1999:
Vol. 286, Issue 5443, pp. 1371-1374
DOI: 10.1126/science.286.5443.1371

You are currently viewing the abstract.

View Full Text

Abstract

Control of messenger RNA (mRNA) stability serves as an important mechanism for regulating gene expression. Analysis ofArabidopsis mutants that overaccumulate soluble methionine (Met) revealed that the gene for cystathionine γ-synthase (CGS), the key enzyme in Met biosynthesis, is regulated at the level of mRNA stability. Transfection experiments with wild-type and mutant forms of the CGS gene suggest that an amino acid sequence encoded by the first exon of CGS acts in cis to destabilize its own mRNA in a process that is activated by Met or one of its metabolites.

  • * Present address: Fukujuen CHA Research Center, Kyoto 619-0223, Japan.

  • Present address: Laboratory of Molecular Phylogenetics, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan.

  • To whom correspondence should be addressed. E-mail: naito{at}abs.agr.hokudai.ac.jp

View Full Text