Evidence for Autoregulation of Cystathionine γ-Synthase mRNA Stability in Arabidopsis

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Science  12 Nov 1999:
Vol. 286, Issue 5443, pp. 1371-1374
DOI: 10.1126/science.286.5443.1371

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Control of messenger RNA (mRNA) stability serves as an important mechanism for regulating gene expression. Analysis ofArabidopsis mutants that overaccumulate soluble methionine (Met) revealed that the gene for cystathionine γ-synthase (CGS), the key enzyme in Met biosynthesis, is regulated at the level of mRNA stability. Transfection experiments with wild-type and mutant forms of the CGS gene suggest that an amino acid sequence encoded by the first exon of CGS acts in cis to destabilize its own mRNA in a process that is activated by Met or one of its metabolites.

  • * Present address: Fukujuen CHA Research Center, Kyoto 619-0223, Japan.

  • Present address: Laboratory of Molecular Phylogenetics, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan.

  • To whom correspondence should be addressed. E-mail: naito{at}

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