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Phosphorylation and Regulation of Raf by Akt (Protein Kinase B)

Science  26 Nov 1999:
Vol. 286, Issue 5445, pp. 1741-1744
DOI: 10.1126/science.286.5445.1741

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Abstract

Activation of the protein kinase Raf can lead to opposing cellular responses such as proliferation, growth arrest, apoptosis, or differentiation. Akt (protein kinase B), a member of a different signaling pathway that also regulates these responses, interacted with Raf and phosphorylated this protein at a highly conserved serine residue in its regulatory domain in vivo. This phosphorylation of Raf by Akt inhibited activation of the Raf-MEK-ERK signaling pathway and shifted the cellular response in a human breast cancer cell line from cell cycle arrest to proliferation. These observations provide a molecular basis for cross talk between two signaling pathways at the level of Raf and Akt.

  • * To whom correspondence should be addressed. E-mail: moelling{at}immv.unizh.ch

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