Requirement of Yeast SGS1 and SRS2 Genes for Replication and Transcription

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Science  17 Dec 1999:
Vol. 286, Issue 5448, pp. 2339-2342
DOI: 10.1126/science.286.5448.2339

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The SGS1 gene of the yeastSaccharomyces cerevisiae encodes a DNA helicase with homology to the human Bloom's syndrome gene BLM and the Werner's syndrome gene WRN. The SRS2 gene of yeast also encodes a DNA helicase. Simultaneous deletion ofSGS1 and SRS2 is lethal in yeast. Here, using a conditional mutation of SGS1, it is shown that DNA replication and RNA polymerase I transcription are drastically inhibited in the srs2Δ sgs1-ts strain at the restrictive temperature. Thus, SGS1 and SRS2function in DNA replication and RNA polymerase I transcription. These functions may contribute to the various defects observed in Werner's and Bloom's syndromes.

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