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Creating a Protein-Based Element of Inheritance

Science  28 Jan 2000:
Vol. 287, Issue 5453, pp. 661-664
DOI: 10.1126/science.287.5453.661

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Abstract

Proteins capable of self-perpetuating changes in conformation and function (known as prions) can serve as genetic elements. To test whether novel prions could be created by recombinant methods, a yeast prion determinant was fused to the rat glucocorticoid receptor. The fusion protein existed in different heritable functional states, switched between states at a low spontaneous rate, and could be induced to switch by experimental manipulations. The complete change in phenotype achieved by transferring a prion determinant from one protein to another confirms the protein-only nature of prion inheritance and establishes a mechanism for engineering heritable changes in phenotype that should be broadly applicable.

  • * To whom correspondence should be addressed. E-mail: S-Lindquist{at}uchicago.edu

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