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Polarization of Chemoattractant Receptor Signaling During Neutrophil Chemotaxis

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Science  11 Feb 2000:
Vol. 287, Issue 5455, pp. 1037-1040
DOI: 10.1126/science.287.5455.1037

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Abstract

Morphologic polarity is necessary for chemotaxis of mammalian cells. As a probe of intracellular signals responsible for this asymmetry, the pleckstrin homology domain of the AKT protein kinase (or protein kinase B), tagged with the green fluorescent protein (PHAKT-GFP), was expressed in neutrophils. Upon exposure of cells to chemoattractant, PHAKT-GFP is recruited selectively to membrane at the cell's leading edge, indicating an internal signaling gradient that is much steeper than that of the chemoattractant. Translocation of PHAKT-GFP is inhibited by toxin-B from Clostridium difficile, indicating that it requires activity of one or more Rho guanosine triphosphatases.

  • * These authors contributed equally to this work.

  • To whom correspondence should be addressed. E-mail: bourne{at}cmp.ucsf.edu

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