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Roles of PLC-β2 and -β3 and PI3Kγ in Chemoattractant-Mediated Signal Transduction

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Science  11 Feb 2000:
Vol. 287, Issue 5455, pp. 1046-1049
DOI: 10.1126/science.287.5455.1046

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Abstract

The roles of phosphoinositide 3-kinase (PI3K) and phospholipase C (PLC) in chemoattractant-elicited responses were studied in mice lacking these key enzymes. PI3Kγ was required for chemoattractant-induced production of phosphatidylinositol 3,4,5-trisphosphate [PtdIns (3,4,5)P3] and has an important role in chemoattractant-induced superoxide production and chemotaxis in mouse neutrophils and in production of T cell–independent antigen-specific antibodies composed of the immunoglobulin λ light chain (TI-IgλL). The study of the mice lacking PLC-β2 and -β3 revealed that the PLC pathways have an important role in chemoattractant-mediated production of superoxide and regulation of protein kinases, but not chemotaxis. The PLC pathways also appear to inhibit the chemotactic activity induced by certain chemoattractants and to suppress TI-IgλL production.

  • * These authors contributed equally to this work.

  • Present address: Department of Pharmacology, Boehringer Inglheim Pharmaceuticals, Inc., Ridgefield, CT 06877, USA.

  • To whom correspondence should be addressed. E-mail: dwu{at}neuron.uchc.edu

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