Report

An Ultrasensitive Bacterial Motor Revealed by Monitoring Signaling Proteins in Single Cells

Science  03 Mar 2000:
Vol. 287, Issue 5458, pp. 1652-1655
DOI: 10.1126/science.287.5458.1652

You are currently viewing the abstract.

View Full Text

Via your Institution

Log in through your institution

Log in through your institution


Abstract

Understanding biology at the single-cell level requires simultaneous measurements of biochemical parameters and behavioral characteristics in individual cells. Here, the output of individual flagellar motors in Escherichia coli was measured as a function of the intracellular concentration of the chemotactic signaling protein. The concentration of this molecule, fused to green fluorescent protein, was monitored with fluorescence correlation spectroscopy. Motors from different bacteria exhibited an identical steep input-output relation, suggesting that they actively contribute to signal amplification in chemotaxis. This experimental approach can be extended to quantitative in vivo studies of other biochemical networks.

  • * To whom correspondence should be addressed. E-mail: phcluzel{at}phoenix.princeton.edu

View Full Text

Related Content