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A BAC-Based Physical Map of the Major Autosomes of Drosophila melanogaster

Science  24 Mar 2000:
Vol. 287, Issue 5461, pp. 2271-2274
DOI: 10.1126/science.287.5461.2271

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Abstract

We constructed a bacterial artificial chromosome (BAC)–based physical map of chromosomes 2 and 3of Drosophila melanogaster, which constitute 81% of the genome. Sequence tagged site (STS) content, restriction fingerprinting, and polytene chromosome in situ hybridization approaches were integrated to produce a map spanning the euchromatin. Three of five remaining gaps are in repeat-rich regions near the centromeres. A tiling path of clones spanning this map and STS maps of chromosomesX and 4 was sequenced to low coverage; the maps and tiling path sequence were used to support and verify the whole-genome sequence assembly, and tiling path BACs were used as templates in sequence finishing.

  • * To whom correspondence should be addressed. E-mail: hoskins{at}bdgp.lbl.gov

  • Present address: Children's Hospital Oakland Research Institute, Oakland, CA 94609, USA.

  • Present address: Parke Davis Laboratory for Molecular Genetics, Alameda, CA 94502, USA.

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