Research Article

Structure of the S15,S6,S18-rRNA Complex: Assembly of the 30S Ribosome Central Domain

See allHide authors and affiliations

Science  07 Apr 2000:
Vol. 288, Issue 5463, pp. 107-112
DOI: 10.1126/science.288.5463.107

You are currently viewing the abstract.

View Full Text

Log in to view the full text

Log in through your institution

Log in through your institution


The crystal structure of a 70-kilodalton ribonucleoprotein complex from the central domain of the Thermus thermophilus30S ribosomal subunit was solved at 2.6 angstrom resolution. The complex consists of a 104-nucleotide RNA fragment composed of two three-helix junctions that lie at the end of a central helix, and the ribosomal proteins S15, S6, and S18. S15 binds the ribosomal RNA early in the assembly of the 30S ribosomal subunit, stabilizing a conformational reorganization of the two three-helix junctions that creates the RNA fold necessary for subsequent binding of S6 and S18. The structure of the complex demonstrates the central role of S15-induced reorganization of central domain RNA for the subsequent steps of ribosome assembly.

  • * These authors contributed equally to this work.

  • To whom correspondence should be addressed. E-mail: dave{at}, jrwill{at}

View Full Text