Research Article

Structure of the S15,S6,S18-rRNA Complex: Assembly of the 30S Ribosome Central Domain

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Science  07 Apr 2000:
Vol. 288, Issue 5463, pp. 107-112
DOI: 10.1126/science.288.5463.107

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Abstract

The crystal structure of a 70-kilodalton ribonucleoprotein complex from the central domain of the Thermus thermophilus30S ribosomal subunit was solved at 2.6 angstrom resolution. The complex consists of a 104-nucleotide RNA fragment composed of two three-helix junctions that lie at the end of a central helix, and the ribosomal proteins S15, S6, and S18. S15 binds the ribosomal RNA early in the assembly of the 30S ribosomal subunit, stabilizing a conformational reorganization of the two three-helix junctions that creates the RNA fold necessary for subsequent binding of S6 and S18. The structure of the complex demonstrates the central role of S15-induced reorganization of central domain RNA for the subsequent steps of ribosome assembly.

  • * These authors contributed equally to this work.

  • To whom correspondence should be addressed. E-mail: dave{at}scripps.edu, jrwill{at}scripps.edu

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