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Identification of a Coordinate Regulator of Interleukins 4, 13, and 5 by Cross-Species Sequence Comparisons

Science  07 Apr 2000:
Vol. 288, Issue 5463, pp. 136-140
DOI: 10.1126/science.288.5463.136

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Abstract

Long-range regulatory elements are difficult to discover experimentally; however, they tend to be conserved among mammals, suggesting that cross-species sequence comparisons should identify them. To search for regulatory sequences, we examined about 1 megabase of orthologous human and mouse sequences for conserved noncoding elements with greater than or equal to 70% identity over at least 100 base pairs. Ninety noncoding sequences meeting these criteria were discovered, and the analysis of 15 of these elements found that about 70% were conserved across mammals. Characterization of the largest element in yeast artificial chromosome transgenic mice revealed it to be a coordinate regulator of three genes, interleukin-4,interleukin-13, and interleukin-5, spread over 120 kilobases.

  • * To whom correspondence should be addressed. E-mail: kafrazer{at}lbl.gov (K.A.F.) and emrubin{at}lbl.gov (E.M.R.)

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