Translating Biomolecular Recognition into Nanomechanics

Science  14 Apr 2000:
Vol. 288, Issue 5464, pp. 316-318
DOI: 10.1126/science.288.5464.316

You are currently viewing the abstract.

View Full Text
As a service to the community, AAAS/Science has made this article free with registration.


We report the specific transduction, via surface stress changes, of DNA hybridization and receptor-ligand binding into a direct nanomechanical response of microfabricated cantilevers. Cantilevers in an array were functionalized with a selection of biomolecules. The differential deflection of the cantilevers was found to provide a true molecular recognition signal despite large nonspecific responses of individual cantilevers. Hybridization of complementary oligonucleotides shows that a single base mismatch between two 12-mer oligonucleotides is clearly detectable. Similar experiments on protein A–immunoglobulin interactions demonstrate the wide-ranging applicability of nanomechanical transduction to detect biomolecular recognition.

  • * To whom correspondence should be addressed. E-mail: ge{at}

View Full Text

Cited By...