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Homologs of Small Nucleolar RNAs in Archaea

Science  21 Apr 2000:
Vol. 288, Issue 5465, pp. 517-522
DOI: 10.1126/science.288.5465.517

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Abstract

In eukaryotes, dozens of posttranscriptional modifications are directed to specific nucleotides in ribosomal RNAs (rRNAs) by small nucleolar RNAs (snoRNAs). We identified homologs of snoRNA genes in both branches of the Archaea. Eighteen small sno-like RNAs (sRNAs) were cloned from the archaeon Sulfolobus acidocaldarius by coimmunoprecipitation with archaeal fibrillarin and NOP56, the homologs of eukaryotic snoRNA-associated proteins. We trained a probabilistic model on these sRNAs to search for more sRNAs in archaeal genomic sequences. Over 200 additional sRNAs were identified in seven archaeal genomes representing both the Crenarchaeota and the Euryarchaeota. snoRNA-based rRNA processing was therefore probably present in the last common ancestor of Archaea and Eukarya, predating the evolution of a morphologically distinct nucleolus.

  • * Present address: Department of Genetics, M322, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305–5120, USA.

  • To whom correspondence should be addressed. E-mail: patrick.p.dennis{at}ubc.ca

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