Report

Movement of Retinal Along the Visual Transduction Path

See allHide authors and affiliations

Science  23 Jun 2000:
Vol. 288, Issue 5474, pp. 2209-2212
DOI: 10.1126/science.288.5474.2209

You are currently viewing the abstract.

View Full Text

Abstract

Movement of the ligand/receptor complex in rhodopsin (Rh) has been traced. Bleaching of diazoketo rhodopsin (DK-Rh) containing 11-cis-3-diazo-4-oxo-retinal yields batho-, lumi-, meta-I-, and meta-II-Rh intermediates corresponding to those of native Rh but at lower temperatures. Photoaffinity labeling of DK-Rh and these bleaching intermediates shows that the ionone ring cross-links to tryptophan-265 on helix F in DK-Rh and batho-Rh, and to alanine-169 on helix D in lumi-, meta-I-, and meta-II-Rh intermediates. It is likely that these movements involving a flip-over of the chromophoric ring trigger changes in cytoplasmic membrane loops resulting in heterotrimeric guanine nucleotide–binding protein (G protein) activation.

  • * To whom correspondence should be addressed. E-mail: kn5{at}columbia.edu

View Full Text