Detection of NO

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Science  07 Jul 2000:
Vol. 289, Issue 5476, pp. 15
DOI: 10.1126/science.289.5476.15b

Nitrous oxide (NO) is implicated in many metabolic processes, yet current methods for in vivo detection of NO rely on measuring metabolites such as nitrite, nitrate, or NOx species. Franz et al. have now synthesized a transition metal complex which directly detects NO in the range of 100 mM. In the “off” state, four nitrogen ligands are bound to the central cobalt atom, and virtually no fluorescent signal is observed. Both quenching by the cobalt atom and excimer complex formation in the stacked fluorescent ligands may contribute to suppression of fluorescence. Replacing two of the imine nitrogens with NO molecules triggers a fluorescent response because one of the dansyl groups is displaced; this fluorescence may be enhanced by formation of a d10 Co-dinitrosyl species. This sensor does not react with O2 and may lead to a more sensitive and reversible sensor for in vivo NO detection. — JU

Angew. Chem. Int. Ed. 39, 2120 (2000).

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