Induction of Mating in Candida albicans by Construction of MTLa and MTLα Strains

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Science  14 Jul 2000:
Vol. 289, Issue 5477, pp. 310-313
DOI: 10.1126/science.289.5477.310

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Although the diploid fungus Candida albicans, a human pathogen, has been thought to have no sexual cycle, it normally possesses mating-type–like orthologs (MTL) of both of theSaccharomyces cerevisiae mating-type genes (MAT) a and α. When strains containing only MTL a or MTLα were constructed by the loss of one homolog of chromosome 5, the site of theMTL loci, MTL a and MTLα strains mated, but like mating types did not. Evidence for mating included formation of stable prototrophs from strains with complementing auxotrophic markers; these contained both MTLalleles and molecular markers from both parents and were tetraploid in DNA content and mononucleate.

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