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Pol κ: A DNA Polymerase Required for Sister Chromatid Cohesion

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Science  04 Aug 2000:
Vol. 289, Issue 5480, pp. 774-779
DOI: 10.1126/science.289.5480.774

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Abstract

Establishment of cohesion between sister chromatids is coupled to replication fork passage through an unknown mechanism. Here we report that TRF4, an evolutionarily conserved gene necessary for chromosome segregation, encodes a DNA polymerase with β-polymerase–like properties. A double mutant in the redundant homologs, TRF4 and TRF5, is unable to complete S phase, whereas a trf4 single mutant completes a presumably defective S phase that results in a failure of cohesion between the replicated sister chromatids. This suggests that TRFs are a key link in the coordination between DNA replication and sister chromatid cohesion. Trf4 and Trf5 represent the fourth class of essential nuclear DNA polymerases (designated DNA polymerase kappa) inSaccharomyces cerevisiae and probably in all eukaryotes.

  • * Present address: Johns Hopkins University, Department of Molecular Biology and Genetics, 725 North Wolfe Street, 504 PCTB, Baltimore, MD 21205–2185, USA.

  • To whom correspondence should be addressed. E-mail: mfc3f{at}virginia.edu

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