Cell Biology

Rebuilding the Network

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Science  06 Oct 2000:
Vol. 290, Issue 5489, pp. 13
DOI: 10.1126/science.290.5489.13c

The nucleus is surrounded by a double membrane, the nuclear envelope, which is in turn lined by a network of proteins known as lamins. In mammalian cells, the nuclear envelope and the nuclear lamin network must disassemble to allow mitosis to proceed, and then reassemble.

Steen et al. examined the requirements for nuclear reassembly and discovered that the protein AKAP149 (A-kinase anchoring protein 149) is important in recruiting lamin B to the re-formed nuclear envelope in interphase cell extracts. In addition to binding the A-kinase, AKAP149 also binds protein phosphatase type 1 (PP1), which dovetails with the modulation of organelle structure by phosphorylation and dephosphorylation cascades during mitosis. Understanding how these cascades control the localization of key enzymes that contribute to the changes in cellular architecture before, during, and after mitosis can now be studied in reconstituted systems that faithfully reproduce intracellular processes. — SMH

J. Cell Biol.150, 1251 (2000).

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