VirB/D4-Dependent Protein Translocation from Agrobacterium into Plant Cells

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Science  03 Nov 2000:
Vol. 290, Issue 5493, pp. 979-982
DOI: 10.1126/science.290.5493.979

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The Agrobacterium VirB/D4 transport system mediates the transfer of a nucleoprotein T complex into plant cells, leading to crown gall disease. In addition, several Virulence proteins must somehow be transported to fulfill a function in planta. Here, we used fusions between Cre recombinase and VirE2 or VirF to directly demonstrate protein translocation into plant cells. Transport of the proteins was monitored by a Cre-mediated in planta recombination event resulting in a selectable phenotype and depended on the VirB/D4 transport system but did not require transferred DNA.

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