Cell Biology

Golgi Indissoluble

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Science  10 Nov 2000:
Vol. 290, Issue 5494, pp. 1053
DOI: 10.1126/science.290.5494.1053c

The contributions of the Golgi apparatus to protein maturation and membrane trafficking make it an essential component of eukaryotic cells. Using a microsurgical technique, Pelletier et al. have examined the effects of removing the Golgi by dividing cells into cytoplasts [containing endoplasmic reticulum (ER) but lacking the nucleus-proximal Golgi] and karyoplasts (containing nucleus, Golgi, and ER).

Newly synthesized proteins in the Golgi-free cytoplasts did not arrive at the cell surface, and no apparent regeneration of the Golgi apparatus from the ER was detected. When a portion of Golgi membranes were captured in the cytoplasts, intracellular transport to the plasma membrane did occur. Thus, a distinctive set of Golgi membranes, which cannot be replenished by the ER, appears to be a requirement for moving protein cargoes to the cell surface. In related work, Seemann et al. show that Golgi-resident enzymes can recycle quantitatively to the ER, but that the cell retains matrix proteins that provide the scaffold for reconstructing a functional Golgi apparatus. — SMH

Nature Cell Biol.2, 840 (2000); Nature407, 1022 (2000).

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