Compartmentalized Destruction

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Science  05 Jan 2001:
Vol. 291, Issue 5501, pp. 17
DOI: 10.1126/science.291.5501.17c

Regulated proteolysis contributes to the proper control of many signal transduction pathways. Lenk and Sommer report that the turnover of the yeast transcriptional repressor Matα2 is controlled through an intrinsic degradation motif known as Deg1. The subcellular distribution and half-life of a Deg1 domain fused to green fluorescent protein in the presence or absence of an exogenous nuclear localization sequence was analyzed in wild-type yeast and in mutants that were defective in nuclear transport. Only those green fluorescent protein constructs that were imported into the nucleus and contained an intact Deg1 motif were degraded rapidly; conversely, adding a nuclear export sequence extended the half-life. The data suggest that there is nuclear-specific activity of components of the ubiquitin-proteasome degradation pathway and that subcellular trafficking of a signaling molecule can influence its susceptibility to destruction. — LBR

J. Biol. Chem.275, 39403 (2000).

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