Report

Transgenic Monkeys Produced by Retroviral Gene Transfer into Mature Oocytes

Science  12 Jan 2001:
Vol. 291, Issue 5502, pp. 309-312
DOI: 10.1126/science.291.5502.309

You are currently viewing the abstract.

View Full Text

Via your Institution

Log in through your institution

Log in through your institution


Abstract

Transgenic rhesus monkeys carrying the green fluorescent protein (GFP) gene were produced by injecting pseudotyped replication-defective retroviral vector into the perivitelline space of 224 mature rhesus oocytes, later fertilized by intracytoplasmic sperm injection. Of the three males born from 20 embryo transfers, one was transgenic when accessible tissues were assayed for transgene DNA and messenger RNA. All tissues that were studied from a fraternal set of twins, miscarried at 73 days, carried the transgene, as confirmed by Southern analyses, and the GFP transgene reporter was detected by both direct and indirect fluorescence imaging.

  • * To whom correspondence should be addressed. E-mail: schatten{at}ohsu.edu

View Full Text

Related Content