CELL BIOLOGY: Psychedelic Trafficking

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Science  26 Jan 2001:
Vol. 291, Issue 5504, pp. 557a
DOI: 10.1126/science.291.5504.557a

Visualization of multiple proteins moving in live cells has become possible with the introduction of green fluorescent protein (GFP) variants. Keller et al. used GFP-fusion proteins and sophisticated image analysis to monitor the sorting of proteins upon exit from the Golgi apparatus. They found that cargo protein destined for the apical surface of polarized epithelial cells was segregated from basolateral cargoes in the Golgi and trans-Golgi network. Furthermore, these proteins were transported in separate carriers to their destinations without intersecting with incoming endocytic traffic, which had been suggested in earlier studies. Using total internal reflection microscopy, the authors could image the delivery and fusion of carrier vesicles with both apical and basolateral surfaces, and they observed that particular regions seemed to be particularly likely fusion sites. — SMH

Acknowledgments

Nature. Cell Biol. 3, 140 (2001).

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