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TRP-PLIK, a Bifunctional Protein with Kinase and Ion Channel Activities

Science  09 Feb 2001:
Vol. 291, Issue 5506, pp. 1043-1047
DOI: 10.1126/science.1058519

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Abstract

We cloned and characterized a protein kinase and ion channel, TRP-PLIK. As part of the long transient receptor potential channel subfamily implicated in control of cell division, it is a protein that is both an ion channel and a protein kinase. TRP-PLIK phosphorylated itself, displayed a wide tissue distribution, and, when expressed in CHO-K1 cells, constituted a nonselective, calcium-permeant, 105-picosiemen, steeply outwardly rectifying conductance. The zinc finger containing α-kinase domain was functional. Inactivation of the kinase activity by site-directed mutagenesis and the channel's dependence on intracellular adenosine triphosphate (ATP) demonstrated that the channel's kinase activity is essential for channel function.

  • * To whom correspondence should be addressed. E-mail: clapham{at}rascal.med.harvard.edu

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