A High-Resolution Radiation Hybrid Map of the Human Genome Draft Sequence

Science  16 Feb 2001:
Vol. 291, Issue 5507, pp. 1298-1302
DOI: 10.1126/science.1057437

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We have constructed a physical map of the human genome by using a panel of 90 whole-genome radiation hybrids (the TNG panel) in conjunction with 40,322 sequence-tagged sites (STSs) derived from random genomic sequences as well as expressed sequences. Of 36,678 STSs on the TNG radiation hybrid map, only 3604 (9.8%) were absent from the unassembled draft sequence of the human genome. Of 20,030 STSs ordered on the TNG map as well as the assembled human genome draft sequence and the Celera assembled human genome sequence, 36% of the STSs had a discrepant order between the working draft sequence and the Celera sequence. The TNG map order was identical to one of the two sequence orders in 60% of these discrepant cases.

  • * Present address: Celera Genomics, 45 West Gude Drive, Rockville, MD 20850, USA.

  • Present address: UCSF Comprehensive Cancer Center, 2340 Sutter Street, San Francisco, CA 94143, USA.

  • Present address: Incyte Genomics Inc., 3160 Porter Drive, Palo Alto, CA 94304, USA.

  • § To whom correspondence should be addressed. E-mail: cox{at}

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