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Structural Mechanism of Endosome Docking by the FYVE Domain

Science  02 Mar 2001:
Vol. 291, Issue 5509, pp. 1793-1796
DOI: 10.1126/science.291.5509.1793

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Abstract

The recruitment of trafficking and signaling proteins to membranes containing phosphatidylinositol 3-phosphate [PtdIns(3)P] is mediated by FYVE domains. Here, the solution structure of the FYVE domain of the early endosome antigen 1 protein (EEA1) in the free state was compared with the structures of the domain complexed with PtdIns(3)P and mixed micelles. The multistep binding mechanism involved nonspecific insertion of a hydrophobic loop into the lipid bilayer, positioning and activating the binding pocket. Ligation of PtdIns(3)P then induced a global structural change, drawing the protein termini over the bound phosphoinositide by extension of a hinge. Specific recognition of the 3-phosphate was determined indirectly and directly by two clusters of conserved arginines.

  • * To whom correspondence should be addressed. E-mail: tatiana.kutateladze{at}uchsc.edu

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