Requirement for the SLP-76 Adaptor GADS in T Cell Development

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Science  09 Mar 2001:
Vol. 291, Issue 5510, pp. 1987-1991
DOI: 10.1126/science.1057176

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GADS is an adaptor protein implicated in CD3 signaling because of its ability to link SLP-76 to LAT. A GADS-deficient mouse was generated by gene targeting, and the function of GADS in T cell development and activation was examined. GADSCD4CD8 thymocytes exhibited a severe block in proliferation but still differentiated into mature T cells. GADS thymocytes failed to respond to CD3 cross-linking in vivo and were impaired in positive and negative selection. Immunoprecipitation experiments revealed that the association between SLP-76 and LAT was uncoupled in GADS thymocytes. These observations indicate that GADS is a critical adaptor for CD3 signaling.

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