Report

5'-Deoxyribose Phosphate Lyase Activity of Human DNA Polymerase ɩ in Vitro

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Science  16 Mar 2001:
Vol. 291, Issue 5511, pp. 2156-2159
DOI: 10.1126/science.1058386

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Abstract

DNA polymerase iota (pol ι) is one of several recently discovered DNA polymerases in mammalian cells whose function is unknown. We report here that human pol ι has an intrinsic 5′-deoxyribose phosphate (dRP) lyase activity. In reactions reconstituted with uracil-DNA glycosylase (UDG), apurinic/apyrimidinic (AP) endonuclease and DNA ligase I, pol ι can use its dRP lyase and polymerase activities to repair G•U and A•U pairs in DNA. These data and three distinct catalytic properties of pol ι implicate it in specialized forms of base excision repair (BER).

  • * To whom correspondence should be addressed. E-mail: kunkel{at}niehs.nih.gov

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