5'-Deoxyribose Phosphate Lyase Activity of Human DNA Polymerase ɩ in Vitro

+ See all authors and affiliations

Science  16 Mar 2001:
Vol. 291, Issue 5511, pp. 2156-2159
DOI: 10.1126/science.1058386

You are currently viewing the abstract.

View Full Text


DNA polymerase iota (pol ι) is one of several recently discovered DNA polymerases in mammalian cells whose function is unknown. We report here that human pol ι has an intrinsic 5′-deoxyribose phosphate (dRP) lyase activity. In reactions reconstituted with uracil-DNA glycosylase (UDG), apurinic/apyrimidinic (AP) endonuclease and DNA ligase I, pol ι can use its dRP lyase and polymerase activities to repair G•U and A•U pairs in DNA. These data and three distinct catalytic properties of pol ι implicate it in specialized forms of base excision repair (BER).

  • * To whom correspondence should be addressed. E-mail: kunkel{at}

View Full Text