Report

Reversible Unfolding of Single RNA Molecules by Mechanical Force

Science  27 Apr 2001:
Vol. 292, Issue 5517, pp. 733-737
DOI: 10.1126/science.1058498

You are currently viewing the abstract.

View Full Text

Via your Institution

Log in through your institution

Log in through your institution


Abstract

Here we use mechanical force to induce the unfolding and refolding of single RNA molecules: a simple RNA hairpin, a molecule containing a three-helix junction, and the P5abc domain of theTetrahymena thermophila ribozyme. All three molecules (P5abc only in the absence of Mg2+) can be mechanically unfolded at equilibrium, and when kept at constant force within a critical force range, are bi-stable and hop between folded and unfolded states. We determine the force-dependent equilibrium constants for folding/unfolding these single RNA molecules and the positions of their transition states along the reaction coordinate.

  • * To whom correspondence should be addressed. E-mail: carlos{at}alice.berkeley.edu

  • * To whom correspondence should be addressed. E-mail: jliphard{at}alice.berkeley.gov or JTLiphardt{at}lbl.gov

View Full Text

Related Content