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Genetic Analysis of Digestive Physiology Using Fluorescent Phospholipid Reporters

Science  18 May 2001:
Vol. 292, Issue 5520, pp. 1385-1388
DOI: 10.1126/science.1060418

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Abstract

Zebrafish are a valuable model for mammalian lipid metabolism; larvae process lipids similarly through the intestine and hepatobiliary system and respond to drugs that block cholesterol synthesis in humans. After ingestion of fluorescently quenched phospholipids, endogenous lipase activity and rapid transport of cleavage products results in intense gall bladder fluorescence. Genetic screening identifies zebrafish mutants, such as fat free, that show normal digestive organ morphology but severely reduced phospholipid and cholesterol processing. Thus, fluorescent lipids provide a sensitive readout of lipid metabolism and are a powerful tool for identifying genes that mediate vertebrate digestive physiology.

  • * These authors contributed equally to this work.

  • To whom correspondence should be addressed. E-mail: sfarber{at}lac.jci.tju.edu, mpack{at}mail.med.upenn.edu

  • Present address: Department of Microbiology and Immunology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA.

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