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Telomere Position Effect in Human Cells

Science  15 Jun 2001:
Vol. 292, Issue 5524, pp. 2075-2077
DOI: 10.1126/science.1062329

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Abstract

In yeast, telomere position effect (TPE) results in the reversible silencing of genes near telomeres. Here we demonstrate the presence of TPE in human cells. HeLa clones containing a luciferase reporter adjacent to a newly formed telomere express 10 times less luciferase than do control clones generated by random integration. Luciferase expression is restored by trichostatin A, a histone deacetylase inhibitor. Overexpression of a human telomerase reverse transcriptase complementary DNA results in telomere elongation and an additional 2- to 10-fold decrease in expression in telomeric clones but not control clones. The dependence of TPE on telomere length provides a mechanism for the modification of gene expression throughout the replicative life-span of human cells.

  • * To whom correspondence should be addressed. E-mail: Jerry.Shay{at}UTSouthwestern.edu (J.W.S) or Woodring.Wright{at}UTSouthwestern.edu (W.E.W).

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