Research Article

Crystal Structure of a Carbon Monoxide Dehydrogenase Reveals a [Ni-4Fe-5S] Cluster

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Science  17 Aug 2001:
Vol. 293, Issue 5533, pp. 1281-1285
DOI: 10.1126/science.1061500

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Abstract

The homodimeric nickel-containing CO dehydrogenase from the anaerobic bacterium Carboxydothermus hydrogenoformanscatalyzes the oxidation of CO to CO2. A crystal structure of the reduced enzyme has been solved at 1.6 angstrom resolution. This structure represents the prototype for Ni-containing CO dehydrogenases from anaerobic bacteria and archaea. It contains five metal clusters of which clusters B, B′, and a subunit-bridging, surface-exposed cluster D are cubane-type [4Fe-4S] clusters. The active-site clusters C and C′ are novel, asymmetric [Ni-4Fe-5S] clusters. Their integral Ni ion, which is the likely site of CO oxidation, is coordinated by four sulfur ligands with square planar geometry.

  • * To whom correspondence should be addressed. E-mail: dobbek{at}biochem.mpg.de, vitali.svetlitchnyi{at}uni-bayreuth.de, ortwin.meyer{at}uni-bayreuth.de.

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