Research Article

Patterns of Gene Expression During Drosophila Mesoderm Development

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Science  31 Aug 2001:
Vol. 293, Issue 5535, pp. 1629-1633
DOI: 10.1126/science.1062660

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The transcription factor Twist initiates Drosophilamesoderm development, resulting in the formation of heart, somatic muscle, and other cell types. Using a Drosophila embryo sorter, we isolated enough homozygous twist mutant embryos to perform DNA microarray experiments. Transcription profiles oftwist loss-of-function embryos, embryos with ubiquitoustwist expression, and wild-type embryos were compared at different developmental stages. The results implicate hundreds of genes, many with vertebrate homologs, in stage-specific processes in mesoderm development. One such gene, gleeful, related to the vertebrate Gli genes, is essential for somatic muscle development and sufficient to cause neural cells to express a muscle marker.

  • * Present address: Mail Stop 68-425 Department of Biology, Massachusetts Avenue, Cambridge, MA 02139, USA

  • Present address: Department of Genetics, Sterling Hall of Medicine, 333 Cedar Street, Yale University School of Medicine, New Haven, CT 06520, USA.

  • To whom correspondence should be addressed. E-mail: scott{at}

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